ZEISS Primovert

ZEISS Primovert Specifications

• Microscope Type: The ZEISS Primovert is an inverted microscope, which means that it is designed to observe and image samples from below. This configuration is particularly advantageous for live cell imaging applications, as it allows for the examination of cells growing in culture dishes, flasks, or multiwell plates.

• Observation Method: The Primovert utilizes transmitted light for observation, enabling researchers to visualize specimens through the illumination passing through them. This method is commonly used in brightfield and phase contrast microscopy techniques.

• Contrast Techniques: The microscope offers versatile contrast techniques, including phase contrast, brightfield, and GFP fluorescence. Phase contrast allows for the visualization of transparent or low-contrast specimens, enhancing the visibility of cellular structures. Brightfield microscopy is ideal for observing stained samples or specimens with inherent contrast. Primovert iLED with GFP fluorescence imaging enables the detection and visualization of GFP fluorescently labeled molecules or structures within cells.

• Objectives: The Primovert is compatible with a range of objectives, typically including Plan-Achromat or long working distance LD Plan-Achromat objectives. These objectives offer magnification levels from 4x to 40x and numerical apertures to cater to various imaging requirements, allowing researchers to achieve high-resolution and high-quality images.

• Illumination: The microscope incorporates LED illumination, which provides consistent and adjustable intensity for different contrast techniques. LED illumination offers advantages such as long lifespan, low power consumption, and reduced heat generation.

• Fluorescence: The Primovert iLED is capable of analyzing GFP-labeled cells and quickly switching between phase contrast and fluorecence. This flexibility enables the visualization of various fluorescent labels and markers used in live cell imaging experiments, especially when photobleaching is a concern.

• Stage: The microscope can be equipped with a fixed stage or a motorized stage, providing stability and precise control for sample positioning. The stage may include various sample holders, such as slides, culture dishes, or multiwell plates, to accommodate different experimental setups.

• Sterile Environment Compatibility: To maintain a sterile environment during live cell imaging, the Primovert can be used with a Laminar Flow Box or other environmental chambers. These accessories provide controlled airflow to keep the samples and microscope stage free from contamination, ensuring the viability of cells during prolonged imaging sessions.

• Imaging: The Primovert is designed for digital imaging, offering integration with digital cameras. This allows for the capture of high-quality images and videos of specimens for documentation and analysis. It provides researchers with the ability to document their findings, analyze cellular processes, and share their results efficiently.

• Software: A camera-equipped trinocular or Primovert HDcam is typically accompanied by imaging software, which enables researchers to acquire, process, analyze, and document their images. The software may offer features such as measurement tools, image stitching for large-scale imaging, time-lapse capabilities, and advanced image analysis algorithms like automated cell counting and confluency.

• Ergonomics: The Primovert is designed with user comfort in mind. It incorporates adjustable ergonomic features, such as adjustable viewing angles and comfortable handgrips, to ensure a comfortable and ergonomic working posture during long observation periods.

• Compatible Imaging Accessories: The Primovert is compatible with a range of imaging accessories to enhance its capabilities. These may include incubation chambers for controlling temperature and humidity during live cell imaging, micromanipulators for precise cell manipulation, or time-lapse systems for capturing dynamic cellular processes over time.

For further information and accurate specifications, it is recommended to reach out to a representative from Nushbaum, an authorized Business Partner of ZEISS products. Nushbaum representatives are knowledgeable about ZEISS microscope offerings and can provide expert guidance and support in selecting the right microscope configuration to meet specific research needs.

Please note that specifications may vary depending on the specific model and configuration of the ZEISS Primovert microscope, and it is advisable to consult with a Nushbaum representative for the most up-to-date and detailed information.