ZEISS Primovert: Empowering Live Cell Imaging with Unmatched Precision
The ZEISS Primovert inverted microscope is a powerful tool designed for live cell imaging. Its versatile capabilities allow researchers to switch seamlessly between phase and fluorescence contrast, enabling detailed examination of both unstained and GFP-labeled cells. With the ability to keep cells in a sterile environment even during observation, thanks to compatibility with a Laminar Flow Box, the Primovert ensures optimal conditions for cell viability. The microscope can be equipped with an HDcam and the imaging App Labscope, providing high-quality imaging and convenient data acquisition and analysis. Whether studying cellular processes or conducting time-lapse experiments, the ZEISS Primovert delivers exceptional performance and versatility for live cell imaging applications.
ZEISS Primovert Specifications
Microscope Type: The ZEISS Primovert is an inverted microscope, which means that it is designed to observe and image samples from below. This configuration is particularly advantageous for live cell imaging applications, as it allows for the examination of cells growing in culture dishes, flasks, or multiwell plates.
Observation Method: The Primovert utilizes transmitted light for observation, enabling researchers to visualize specimens through the illumination passing through them. This method is commonly used in brightfield and phase contrast microscopy techniques.
Contrast Techniques: The microscope offers versatile contrast techniques, including phase contrast, brightfield, and GFP fluorescence. Phase contrast allows for the visualization of transparent or low-contrast specimens, enhancing the visibility of cellular structures. Brightfield microscopy is ideal for observing stained samples or specimens with inherent contrast. Primovert iLED with GFP fluorescence imaging enables the detection and visualization of GFP fluorescently labeled molecules or structures within cells.
Objectives: The Primovert is compatible with a range of objectives, typically including Plan-Achromat or long working distance LD Plan-Achromat objectives. These objectives offer magnification levels from 4x to 40x and numerical apertures to cater to various imaging requirements, allowing researchers to achieve high-resolution and high-quality images.
Illumination: The microscope incorporates LED illumination, which provides consistent and adjustable intensity for different contrast techniques. LED illumination offers advantages such as long lifespan, low power consumption, and reduced heat generation.
Fluorescence: The Primovert iLED is capable of analyzing GFP-labeled cells and quickly switching between phase contrast and fluorecence. This flexibility enables the visualization of various fluorescent labels and markers used in live cell imaging experiments, especially when photobleaching is a concern.
Stage: The microscope can be equipped with a fixed stage or a motorized stage, providing stability and precise control for sample positioning. The stage may include various sample holders, such as slides, culture dishes, or multiwell plates, to accommodate different experimental setups.
Sterile Environment Compatibility: To maintain a sterile environment during live cell imaging, the Primovert can be used with a Laminar Flow Box or other environmental chambers. These accessories provide controlled airflow to keep the samples and microscope stage free from contamination, ensuring the viability of cells during prolonged imaging sessions.
Imaging: The Primovert is designed for digital imaging, offering integration with digital cameras. This allows for the capture of high-quality images and videos of specimens for documentation and analysis. It provides researchers with the ability to document their findings, analyze cellular processes, and share their results efficiently.
Software: A camera-equipped trinocular or Primovert HDcam is typically accompanied by imaging software, which enables researchers to acquire, process, analyze, and document their images. The software may offer features such as measurement tools, image stitching for large-scale imaging, time-lapse capabilities, and advanced image analysis algorithms like automated cell counting and confluency.
Ergonomics: The Primovert is designed with user comfort in mind. It incorporates adjustable ergonomic features, such as adjustable viewing angles and comfortable handgrips, to ensure a comfortable and ergonomic working posture during long observation periods.
Compatible Imaging Accessories: The Primovert is compatible with a range of imaging accessories to enhance its capabilities. These may include incubation chambers for controlling temperature and humidity during live cell imaging, micromanipulators for precise cell manipulation, or time-lapse systems for capturing dynamic cellular processes over time.
For further information and accurate specifications, it is recommended to reach out to a representative from Nushbaum, an authorized Business Partner of ZEISS products. Nushbaum representatives are knowledgeable about ZEISS microscope offerings and can provide expert guidance and support in selecting the right microscope configuration to meet specific research needs.
Please note that specifications may vary depending on the specific model and configuration of the ZEISS Primovert microscope, and it is advisable to consult with a Nushbaum representative for the most up-to-date and detailed information.
ZEISS Primovert Description
The ZEISS Primovert inverted microscope combines efficiency and optical performance to offer exceptional imaging capabilities for a wide range of applications. With its advanced features and cutting-edge technology, the Primovert delivers reliable and high-quality results, making it an ideal choice for researchers and scientists engaged in live cell imaging.
The Primovert HDcam comes equipped with an internal camera, ensuring high-definition imaging of cellular structures and dynamic processes. This feature enables researchers to capture detailed and precise images, providing valuable insights into cellular morphology and behavior. The high-resolution imaging capabilities of the HDcam enhance the accuracy and clarity of the observed samples, facilitating in-depth analysis and documentation.
The microscope also features Primovert iLED fluorescence illumination, a powerful LED light source specifically designed for optimal GFP imaging performance. The iLED illumination system delivers consistent and adjustable intensity, providing uniform illumination across the field of view. This ensures accurate and reproducible results, enabling researchers to visualize and study cellular processes with enhanced contrast and clarity.
The Primovert is designed to address a wide range of applications, including but not limited to:
- Cell Biology: The Primovert excels in cell biology research, allowing for the examination of live cells and their dynamic processes. From observing cellular division and migration to studying organelle dynamics and protein localization, the microscope enables researchers to investigate a wide range of cellular phenomena.
- Developmental Biology: The microscope is well-suited for developmental biology studies, enabling the observation of embryonic development, tissue formation, and morphogenesis. The Primovert’s high-resolution imaging capabilities facilitate the detailed examination of intricate developmental processes.
- Stem Cell Research: Stem cell research often requires the observation and characterization of stem cells and their differentiation. The Primovert provides the necessary imaging tools to track and analyze stem cell behavior, allowing researchers to study their potential applications in regenerative medicine and tissue engineering.
- Pharmacology and Drug Discovery: The Primovert is valuable in pharmacological studies and drug discovery, enabling researchers to observe and evaluate the effects of drugs on live cells. The microscope facilitates the investigation of cellular responses, drug toxicity, and drug-target interactions, aiding in the development of new therapeutic approaches.
- Cancer Research: With its advanced imaging capabilities, the Primovert supports cancer research by enabling the visualization and analysis of cancer cells and their behavior, count and measure cell growth and confluency. It allows researchers to study cellular characteristics, proliferation rates, migration patterns, and response to anti-cancer treatments.
The ZEISS Primovert inverted microscope seamlessly combines efficiency and optical performance to meet the demands of a wide range of applications. Its HDcam imaging system, Primovert iLED illumination, and versatile observation techniques provide researchers with a reliable and versatile platform for live cell imaging. Whether investigating cellular processes, developmental biology, stem cells, pharmacology, or cancer research, the Primovert delivers exceptional imaging quality, facilitating breakthrough discoveries and advancements in the field of life sciences.